MAB353A4
Anti-Nestin Antibody, clone rat-401, Alexa Fluor™ 488 conjugate
clone rat-401, from mouse, ALEXA FLUOR™ 488
Synonym(s):
rat401, NES, Nbla00170, FLJ21841
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
rat-401, monoclonal
species reactivity
rat, mouse
should not react with
human
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
Specificity
This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
Immunogen
Embryonic rat spinal cord
Application
Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Quality
Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
Target description
The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Storage and Stability
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Mouse cortical neural stem cells (SCR029)
Mouse cortical neural stem cells (SCR029)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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