ABT1485-AF647
Anti-Actin Antibody, Alexa Fluor™ 647 Conjugate
from rabbit, ALEXA FLUOR™ 647
Synonym(s):
Actin, alpha skeletal muscle, Alpha-actin-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 647
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... ACTA1(58)
General description
Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
Specificity
Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
This Alexa Fluor™ 647 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
Application
Flow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in NIH/3T3 cells.
Immunofluorescence Analysis: A 1:50-150 dilution from a representative lot detected actin in human heart, prostate, skeletal muscle, and small intestine tissue sections.
The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor™ 488-conjugated antibody (Cat. No. ABT1485-AF488) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in NIH/3T3 cells.
Immunofluorescence Analysis: A 1:50-150 dilution from a representative lot detected actin in human heart, prostate, skeletal muscle, and small intestine tissue sections.
The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor™ 488-conjugated antibody (Cat. No. ABT1485-AF488) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Research Category
Cell Structure
Cell Structure
This rabbit polyclonal Anti-Actin, Alexa Fluor™ 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.
Quality
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 cells.
Target description
~42 kDa observed.
Physical form
Affinity purified.
Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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