343781-M

ω-Conotoxin GVIA, Conus geographus

A neurotoxin that acts as an antagonist of voltage-activated N-type Ca²⁺ channels and of neurotransmitter release at neuronal synapses.

• Synonym(s): ω-Conotoxin GVIA, Conus geographus, ω-CgTx GVIA
• Empirical Formula (Hill Notation): C₁₂₀H₁₈₂N₃₈O₄₃S₆
• CAS Number: 106375-28-4
• Molecular Weight: 3037.35
• MDL number: MFCD00076620
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: 250 μg/mL; aqueous buffer: soluble
• storage temp.: −20°C

Description

General description

A neurotoxin that acts as an antagonist of voltage-activated N-type Ca²⁺ channels and of neurotransmitter release at neuronal synapses. Its antagonistic activity is attributed to a tyrosine residue at position 13. Does not bind to dihydropyridine- or verapamil-binding sites. Also reported to inhibit ATP-induced inward currents mediated by P2X2/X3 receptors (IC₅₀ = 21.2 nM for P2X3 and 3.84 µM for P2X2/X3 heterooligomeric receptors).

Biochem/physiol Actions

Primary Target
Voltage-activated N-type Ca2+ channels

Target IC₅₀: 21.2 nM for P2X3 and 3.84 µM for P2X2/X3 heterooligomeric receptors

Warning

Toxicity: Highly Toxic (H)

Sequence

H-Cys¹-Lys-Ser-Hyp-Gly-Ser-Ser-Cys⁸-Ser-Hyp-Thr-Ser-Tyr-Asn-Cys¹⁵-Cys¹⁶-Arg-Ser-Cys¹⁹-Asn-Hyp-Tyr-Thr-Lys-Arg-Cys²⁶-Tyr-NH₂ (disulfide bonds: 1 → 16; 8 → 19; 15 → 26)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Lalo, U.V., et al. 2001. Brain Res. Bull.54, 507.
Kim, J.I., et al. 1995. Biochem. Biophys. Res. Commun. 206, 449.
Abbott, J.R., and Litzinger, M.J. 1994. Int. J. Dev. Neurosci. 12, 43.
Olivera, B.M., et al. 1991. J. Biol. Chem.266, 22067.
Protti, D.A., et al. 1991. Brain Res.557, 336.
Werth, J.L., et al. 1991. Mol. Pharmacol.40, 742.
Koyano, K., et al. 1987. Eur. J. Pharmacol.135, 337.
Abe, T., et al. 1986. Neurosci. Lett.71, 203.

Legal Information

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable