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195235

Sigma-Aldrich

Orange II sodium salt

(Certified by the Biological Stain Commission), Dye content ≥85 %

Synonym(s):

4-(2-Hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt, Acid Orange 7, Acid Orange A, Orange II, Tropaeolin 000 No. 2

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About This Item

Empirical Formula (Hill Notation):
C16H11N2NaO4S
CAS Number:
Molecular Weight:
350.32
Colour Index Number:
15510
Beilstein:
3898201
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product line

BioXtra

form

powder

composition

Dye content, ≥85%

λmax

483 nm

ε (extinction coefficient)

≥17000 at 480-486 nm in water at 0.01 g/L
≥26500 at 225-231 nm in water at 0.01 g/L
≥7500 at 307-313 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3)S([O-])(=O)=O

InChI

1S/C16H12N2O4S.Na/c19-15-10-5-11-3-1-2-4-14(11)16(15)18-17-12-6-8-13(9-7-12)23(20,21)22;/h1-10,19H,(H,20,21,22);/q;+1/p-1/b18-17+;

InChI key

CQPFMGBJSMSXLP-ZAGWXBKKSA-M

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Suitability

Certified for use in the Wilson-Ezrin method for pituitary acidophils, as a counterstain following Heidenhain′s or Weigert′s hematoxylin on paraffin sections, and as a substitute for orange G in Floemming′s triple stain.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ashish Yadav et al.
Toxicology letters, 208(3), 239-245 (2011-12-06)
Orange II, an azo dye, is not permitted in food preparations, but high levels of the dye have been detected in different food commodities. Though there are reports on the toxicity of Orange II but knowledge based on the immunomodulatory
Yan-Qing Wang et al.
Journal of photochemistry and photobiology. B, Biology, 113, 14-21 (2012-05-30)
The interactions between human hemoglobin and orange I (or orange II) were investigated by UV/vis absorption, circular dichroism, fluorescence spectra techniques, and molecular modeling method. Orange I and orange II effectively quenched the intrinsic fluorescence of human hemoglobin by static
Penghui Shi et al.
Journal of hazardous materials, 229-230, 331-339 (2012-06-29)
The current paper investigated the removal of the azo dye Orange II from water using advanced oxidation processes based on sulfate radicals. The cobalt oxide catalyst immobilized on graphene oxide (GO) can activate peroxymonosulfate (PMS) for the degradation of Orange
Huyin Xue et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 29(12), 1840-1848 (2012-08-15)
This paper reports the preparation of polyclonal antibodies against a synthetic azo dye, Orange II, and the development of an indirect ELISA to detect Orange II in foods. The sulfonic group of Orange II was modified and linked with carrier
Waheeba Ahmed Al-Amrani et al.
Bioresource technology, 118, 633-637 (2012-06-19)
The objectives of this study were: (1) to investigate the role of mixed culture of biomass in the regeneration of mono-amine modified silica (MAMS) and granular activated carbon (GAC) loaded with Acid Orange 7 (AO7), (2) to quantify and compare

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