Lysosome Isolation Kit
![Lysosome isolation kit red uptake Lineriaty of uptake with increasing amounts lysosomal protein Neutral red uptake](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/protein-lysis-and-extraction/lysosome-isolation-kit-red-uptake/lysosome-isolation-kit-red-uptake.jpg)
Figure 1.Lineriaty of uptake with increasing amounts lysosomal protein Neutral red uptake
![Lysosome isolation kit blue arrow An example of seperation of lysomes from rat liver on an optiprep gradient: 8 mg protein of the CLF were brought to 19% Optirep concentration and then placed on a 5 mi step gradient. The blue arrows show the approximate position of the Optirep gradient.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/protein-lysis-and-extraction/lysosome-isolation-kit-blue-arrow/lysosome-isolation-kit-blue-arrow.jpg)
Figure 2.An example of seperation of lysomes from rat liver on an optiprep gradient: 8 mg protein of the CLF were brought to 19% Optirep concentration and then placed on a 5 mi step gradient. The blue arrows show the approximate position of the Optirep gradient.
Lysosome Isolation Kit Protocol
![Lysosome Isolation Kit Lysosome Isolation Kit Protocol](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/articles/protein-biology/protein-lysis-and-extraction/lys-flow-chart.gif)
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