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  • Core-shell Fe3O4-Au magnetic nanoparticles based nonenzymatic ultrasensitive electrochemiluminescence immunosensor using quantum dots functionalized graphene sheet as labels.

Core-shell Fe3O4-Au magnetic nanoparticles based nonenzymatic ultrasensitive electrochemiluminescence immunosensor using quantum dots functionalized graphene sheet as labels.

Analytica chimica acta (2013-03-19)
Weiyan Liu, Yan Zhang, Shenguang Ge, Xianrang Song, Jiadong Huang, Mei Yan, Jinghua Yu
RESUMEN

In this paper, a novel, low-cost electrochemiluminescence (ECL) immunosensor using core-shell Fe3O4-Au magnetic nanoparticles (AuMNPs) as the carriers of the primary antibody of carbohydrate antigen 125 (CA125) was designed. Graphene sheet (GS) with property of good conductivity and large surface area was a captivating candidate to amplify ECL signal. We successively synthesized functionalized GS by loading large amounts of quantum dots (QDs) onto the poly (diallyldimethyl-ammonium chloride) (PDDA) coated graphene sheet (P-GS@QDs) via self-assembly electrostatic reactions, which were used to label secondary antibodies. The ECL immunosensors coupled with a microfluidic strategy exhibited a wide detection range (0.005-50 U mL(-1)) and a low detection limit (1.2 mU mL(-1)) with the help of an external magnetic field to gather immunosensors. The method was evaluated with clinical serum sample, receiving good correlation with results from commercially available analytical procedure.

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Sigma-Aldrich
Iron(III) oxide, powder, <5 μm, ≥96%
Sigma-Aldrich
Iron(III) oxide, nanopowder, <50 nm particle size (BET)
Sigma-Aldrich
Iron(III) oxide, ≥99.995% trace metals basis