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Production of Glycolipid Biosurfactants, cellobiose lipids, by Cryptococcus humicola JCM 1461 and their interfacial properties.

Bioscience, biotechnology, and biochemistry (2011-08-09)
Tomotake Morita, Yuko Ishibashi, Tokuma Fukuoka, Tomohiro Imura, Hideki Sakai, Masahiko Abe, Dai Kitamoto
RESUMEN

Cryptococcus humicola JCM 1461 efficiently produced cellobiose lipids (CLs), bolaform biosurfactants. The main product was identified as 16-O-(2″,3″,4″,6'-tetra-O-acetyl-β-cellobiosyl)-2-hydroxyhexadecanoic acid. The production yield of CLs reached 13.1 g/L under the intermittent feeding of glucose. The critical micelle concentrations (CMC) of the main product at pH 4.0 and 7.0 were 3.3×10(-5) M and 4.1×10(-4) M respectively.

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Sigma-Aldrich
2-Hydroxyhexadecanoic acid, ≥98% (capillary GC)