- Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae.
Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae.
Bio-protocol (2018-02-13)
Sviatlana Shashkova, Adam Jm Wollman, Stefan Hohmann, Mark C Leake
PMID29430484
RESUMEN
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here we present a method to characterise maturation of GFP and mCherry genetic protein fusions in budding yeast Saccharomyces cerevisiae.
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Millipore
Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate, suitable for microbiology, NutriSelect® Basic