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Simple transport and cryopreservation of cold-stored mouse embryos.

Experimental animals (2020-06-20)
Ayumi Mukunoki, Toru Takeo, Satohiro Nakao, Kana Tamura, Yuka Horikoshi, Naomi Nakagata
RESUMEN

The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.

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Pyrex® Lifetime Red graduated tube, O.D. × L 25 mm × 200 mm, volume 50.0 mL, accuracy: 0.4 mL