G005815

GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean^™ ENVI-Carb^™ II/PSA

application for SPE, suitable for GC

Propiedades

• technique(s): gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: 10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms); SPE tube/cartridge: Supelclean ENVI-Carb II/PSA SPE Tube (54067-U); condition: 5 mL acetone:toluene (65:35); sample addition: 1 mL sample extract; elution: 10 mL of acetone:toluene (65:35); eluate post-treatment: Evaporate fraction to 0.5 mL then adjust volume to 1 mL with ethyl acetate for GC-NPD analysis; column: SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U); oven: 110 °C (2 min), 2.5 °C/min to 205 °C, 10 °C/min to 310 °C (10 min); inj. temp.: 250 °C; detector: NPD, 310 °C; carrier gas: helium, 0.9 mL/min constant flow; injection: 2 μL, splitless; liner: 4 mm I.D. dual tapered
• suitability: application for SPE
• application(s): agriculture; food and beverages

Descripción

Preparation Note

Sample pretreatment: To a 10 g sample of homogenized food (green onions, apples, Napa cabbage, button mushrooms) was added 10 mL acetonitrile and 4 g magnesium sulfate (that had been heated at 550 °C) and 1 g sodium chloride. After centrifugation, the supernatant was removed and mixed with 1 g magnesium sulfate and centrifuged. 5 mL of the supernatant was removed and evaporated to 1 mL for SPE loading.

Analysis Note

Organophosphorous pesticide use in agriculture is widespread due to the fact that they are more amenable to environmental degradation in comparison to organochlorine or organonitrogen compounds. There are a large number of pesticides in the organophosphorous group, and because of their potential health effects, they are of particular concern on produce imported from areas in which they are commonly used. This chromatogram illustrates the separation of 63 of these pesticides by GC on the SLB-5ms using nitrogen phosphorus detection (NPD).

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analito

Methamidophos
Dichlorvos
Acephate
Omethoate
Ethoprophos
Dicrotophos
Cadusafos
Monocrotophos
Phorate
Terbufos
Propetamphos
Disulfoton
Chloropyrifos-methyl
Parathion-methyl
O,O-Diethyl O-(4-nitrophenyl) phosphate
Malathion
Fenthion
Parathion
Isocarbophos
Trichloronate
Methidathion
Fensulfothion
Ethion
Famphur
Pyridaphenthion
Phosmet
Phosalone
Azinphos-methyl
Azinphos-ethyl
Coumaphos
Temephos
Diazinon
Dichlofenthion
Dimethoate
Phosfolan
Fenitrothion
Phenthoate
Ver todo (37)