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T1074

Sigma-Aldrich

Tricine

Biotechnology Performance Certified, 1 M in solution, suitable for cell culture, 0.2 μm filtered

Sinónimos:

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Fórmula lineal:
(HOCH2)3CNHCH2CO2H
Número de CAS:
5704-04-1
Peso molecular:
179.17
Beilstein/REAXYS Number:
1937804
MDL number:
MFCD00004277
UNSPSC Code:
12161700
PubChem Substance ID:
24899930

grade

Biotechnology Performance Certified

sterility

0.2 μm filtered

concentration

1 M in solution

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
<5 ppm heavy metals as (Pb)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

cation traces

Fe: <5 ppm

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Application

Buffer component for separation of low molecular weight peptides.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

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Syed R Haider et al.
Journal of separation science, 34(18), 2463-2467 (2011-08-06)
This paper compares different buffer systems for the electrophoretic separation of the five most abundant serum proteins on native-PAGE gel and cellulose membranes. A modified Tris-tricine system was shown to be superior for the separation of these serum proteins in
Lindsay M McCracken et al.
Neuropharmacology, 58(3), 676-681 (2009-11-17)
Glycine receptor function mediates most inhibitory neurotransmission in the brainstem and spinal cord and is enhanced by alcohols, volatile anesthetics, inhaled drugs of abuse, and endogenous compounds including zinc. Because zinc exists ubiquitously throughout the brain, investigations of its effects
Arpita Gantayet et al.
Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
Aleksandr Shcheglovitov et al.
The Journal of general physiology, 139(3), 219-234 (2012-03-01)
Here, we describe a new mechanism by which glutamate (Glu) and trace metals reciprocally modulate activity of the Ca(v)2.3 channel by profoundly shifting its voltage-dependent gating. We show that zinc and copper, at physiologically relevant concentrations, occupy an extracellular binding
Syed R Haider et al.
Methods in molecular biology (Clifton, N.J.), 869, 81-91 (2012-05-16)
Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low-molecular-mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins require to be recovered from the gel for quantitative
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