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Merck

11585738910

Roche

DIG-labeled Control DNA

greener alternative

solution, pkg of 50 μL (5 μg/ml DIG-labeled plasmid DNA)

Sinónimos:

control DNA

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About This Item

UNSPSC Code:
41105330

form

solution

packaging

pkg of 50 μL (5 μg/ml DIG-labeled plasmid DNA)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

storage temp.

−20°C

General description

Determination of the yield of digoxigenin (DIG)-labeled DNA is most important for optimal and reproducible hybridization results. Too high of a probe concentration in the hybridization step causes background, while too low of a concentration leads to weak signals.
DIG-labeled control has a standardized concentration and can be used as a standard for comparison to experimentally generated DIG-labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

Estimation of the yield of DIG-labeled nucleic acids or as a control-hybridization probe in Southern blots.

Quality

0.1pg of the DIG-labeled Control DNA can be visualized in a spot test with direct color detection.

Physical form

Solution, digoxigenin-labeled pBR328 DNA, 5 μg/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

No data available

flash_point_c

No data available


Certificados de análisis (COA)

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Artículos

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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