FCMAB113A4
Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor™ 488 conjugate
clone 10H11.2, from mouse, ALEXA FLUOR™ 488
Sinónimos:
Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
ALEXA FLUOR™ 488
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
10H11.2, monoclonal
reactividad de especies
human
técnicas
flow cytometry: suitable
immunocytochemistry: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... POU5F1(5460)
Descripción general
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Especificidad
Reacts with human Oct-4.
Inmunógeno
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Aplicación
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Calidad
Evaluated by Flow Cytometry in 2102Ep Cells.
Descripción de destino
~44 kDa
Forma física
Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Almacenamiento y estabilidad
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
Nota de análisis
Control
Human embryonic stem cells
Human embryonic stem cells
Información legal
ALEXA FLUOR is a trademark of Life Technologies
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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