HPLC Analysis of Phenyl Urea Pesticides on Ascentis® Express C18 Phase
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis® Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)
mobile phase
[A] 0.025 M potassium phosphate buffer, adj. to pH = 2.5, [B] acetonitrile; (50:50, A:B)
flow rate
2.0 mL/min
pressure
300 bar
column temp.
30 °C
detector
UV 245 nm, VWD
injection
0.5 μL
sample
acetonitrile
Description
General description
This separation illustrates the use of the highly efficient 2.7 um Ascentis Express Fused-Core C18 stationary phase in the analysis of some popular herbicides. The short run times allow analyses using isocratic conditions so that column equilibration time is not required between runs.
Other Notes
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany