HPLC Analysis of Conjugated Estrogens on Discovery® HS C18
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
17β-Estradiol solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Estriol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Estrone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Discovery HS C18, 15 cm × 4.6 mm I.D., 5 μm particles (568522-U)
mobile phase
[A] 10 mM potassium phosphate (pH 7.01 unadjusted); [B] acetonitrile; [C] methanol
gradient
80% A, 15% B, 5% C, held for 5 min; to 40% A, 45% B, 15% C in 20 min; held for 5 min
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 220 nm
injection
10 μL
sample
10 μg/mL each in 60/40, water:acetonitrile
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany