HPLC Analysis of Aldehydes and Ketones as DNPH Derivatives on Ascentis® Express C18
Materials
analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)
mobile phase
[A] water; [B] acetonitrile; (40:60, A:B)
flow rate
2 mL/min
pressure
4685 psi (323 bar)
column temp.
30 °C
detector
UV, 360 nm
injection
5 μL
sample
Sample in water
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the separation of aldehyde/ketone-DNPH derivatives.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany