- Different co-culture systems have the same impact on bovine embryo transcriptome.
Different co-culture systems have the same impact on bovine embryo transcriptome.
Reproduction (Cambridge, England) (2017-10-07)
A Vitorino Carvalho, E Canon, L Jouneau, C Archilla, L Laffont, M Moroldo, S Ruffini, E Corbin, P Mermillod, V Duranthon
PMID28982934
ABSTRACT
During the last few years, several co-culture systems using either BOEC or VERO feeder cells have been developed to improve bovine embryo development and these systems give better results at high oxygen concentration (20%). In parallel, the SOF medium, used at 5% O
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
Heparin sodium salt from porcine intestinal mucosa, Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Gentamicin solution, 10 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
BME Amino Acids Solution 50×, Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Fetal Bovine Serum, Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture