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  • Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Food chemistry (2014-05-20)
O Kenny, T J Smyth, D Walsh, C T Kelleher, C M Hewage, N P Brunton
ABSTRACT

Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium minus, Taraxacum officinale, Centaurea nigra and Cirsium palustre demonstrated antimicrobial activity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365μg/ml). Ethanol extracts also had higher antioxidant activities and phenolic content demonstrating a link between these compounds and the bioactivity of these extracts. Further investigation into the phenolic content of ethanol extracts using UPLC-MS/MS lead to the identification and quantification of numerous phenolic compounds in all species including; 18 from Cirsium arvense, 16 from Cirsium vulgare, 19 from C. palustre, 15 from C. nigra, 17 from C. scabiosa, 14 from Sonchus asper, 17 from A. minus and 11 from T. officinale.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Vanillic acid, ≥97%, FG
Supelco
L-Ascorbic acid, analytical standard
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Supelco
Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
Capsaicin, natural
Supelco
(−)-Epicatechin, analytical standard
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
Vanillic acid, 97%
Sigma-Aldrich
Rosmarinic acid, 96%
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
Sigma-Aldrich
Ethyl gallate, antioxidant, ≥96.0% (HPLC)
Sigma-Aldrich
D-(−)-Quinic acid, 98%
Sigma-Aldrich
Sodium carbonate, anhydrous, powder, 99.999% trace metals basis
Supelco
Apigenin, analytical standard
Sigma-Aldrich
L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
L-Ascorbic acid, FCC, FG
Rosmarinic acid, primary reference standard
Chlorogenic acid, primary reference standard
Supelco
Sinapic acid, matrix substance for MALDI-MS, ≥99.5%, Ultra pure
Supelco
Procyanidin B2, analytical standard
Supelco
Capsaicin, analytical standard
USP
Capsaicin, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Apigenin, ≥95.0% (HPLC)
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
USP
Dehydrated Alcohol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
(−)-Epicatechin, ≥98% (HPLC), from green tea
Sigma-Aldrich
(−)-Epicatechin, ≥90% (HPLC)
Sigma-Aldrich
Sodium carbonate, BioXtra, ≥99.0%