- In situ determination of Clostridium endospore membrane fluidity during pressure-assisted thermal processing in combination with nisin or reutericyclin.
In situ determination of Clostridium endospore membrane fluidity during pressure-assisted thermal processing in combination with nisin or reutericyclin.
Applied and environmental microbiology (2013-01-22)
S Hofstetter, R Winter, L M McMullen, M G Gänzle
PMID23335780
ABSTRACT
This study determined the membrane fluidity of clostridial endospores during treatment with heat and pressure with nisin or reutericyclin. Heating (90°C) reduced laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) general polarization, corresponding to membrane fluidization. Pressure (200 MPa) stabilized membrane order. Reutericyclin and nisin exhibit divergent effects on heat- and pressure-induced spore inactivation and membrane fluidity.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, suitable for fluorescence, ≥97.0% (HPLC)