Gold-mediated selective cysteine modification of peptides using allenes.

Gold-mediated selective cysteine modification of peptides using allenes.

Chemical communications (Cambridge, England) (2013-01-17)

Anna On-Yee Chan, Johnson Lui-Lui Tsai, Vanessa Kar-Yan Lo, Gai-Li Li, Man-Kin Wong, Chi-Ming Che

PMID23322001

ABSTRACT

A new approach for selective modification of cysteine-containing peptides through gold-mediated oxidative allene-thiol coupling reaction in aqueous medium is developed.

MATERIALS

Product Number

Brand

Product Description

R5000

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein

R4001

Sigma-Aldrich

Ribonuclease A-agarose, ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)

R4875

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein

R6513

Sigma-Aldrich

Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R5250

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein

R5125

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

R5503

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

R4642

Sigma-Aldrich

Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

R7884

Sigma-Aldrich

Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

R5500

Sigma-Aldrich

Ribonuclease A from bovine pancreas, Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein