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  • Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath.

Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath.

Science advances (2021-09-16)
Xingjie Ren, Mengchi Wang, Bingkun Li, Kirsty Jamieson, Lina Zheng, Ian R Jones, Bin Li, Maya Asami Takagi, Jerry Lee, Lenka Maliskova, Tsz Wai Tam, Miao Yu, Rong Hu, Lindsay Lee, Armen Abnousi, Gang Li, Yun Li, Ming Hu, Bing Ren, Wei Wang, Yin Shen
ABSTRACT

[Figure: see text].

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Doxycycline hyclate
Sigma-Aldrich
Anti-CTCF Antibody, serum, Upstate®
Sigma-Aldrich
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal, culture supernatant, clone MC315, Upstate®
Sigma-Aldrich
6-Thioguanine, Hybri-Max, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma