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  • Rapid induction of edible lipids in Chlorella by mild electric stimulation.

Rapid induction of edible lipids in Chlorella by mild electric stimulation.

Bioresource technology (2019-08-10)
Sun-A Choi, Soo Youn Lee, Jiye Lee, Jun Muk Cho, Jin-Suk Lee, Seung Wook Kim, Dong-Yeon Kim, Se-Kook Park, Chang-Soo Jin, You-Kwan Oh
ABSTRACT

In this work, a new stress-based method for rapid induction of triacylglycerol (TAG) and total and polyunsaturated fatty acid accumulations in Chlorella sp. by mild electric stimulation is presented. When a cathodic current of 31 mA (voltage: 4 V) was applied to the algal cells for 4 h, the TAG content of the electro-treated cells was sharply increased to a level 2.1 times that of the untreated control. The contents of the polyunsaturated linoleic (C18:2n6) and linolenic (C18:3n3) acids in the electro-treated cells were also 36 and 57% higher than those in the untreated cells, respectively. Cyclic voltammetry and various biochemical analyses indicate that TAG and fatty acid formations are electro-stimulated via de novo fatty acid biosynthesis and metabolic transformation in the Chlorella cells.

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Product Description

Corning® 96 Well TC-Treated Microplates, size 96 wells, clear, polystyrene, flat bottom, case of 50 (10 Bulk Packs of 5), sterile, lid