ZRT1327

Anti-LAG3 Antibody, clone C9B7W ZooMAb^® Rat Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, CD223, Lymphocyte activation gene 3 protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rat
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: C9B7W, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using protein G
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• isotype: IgG1
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_032505
• UniProt accession no.: Q61790
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone C9B7W is a ZooMAb^® rat recombinant monoclonal antibody that specifically detects LAG-3. It targets an epitope with the D2 domain from the extracellular region.

Immunogen

Recombinant protein corresponding the D2 domain from the extracellular region of mouse LAG3.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Muse LAG3

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant extracellular domain (D1-D4) of Mouse LAG3 fused to the Fc portion of Mouse IgG2a.

Quality Control Testing

Evaluated by Western Blotting with recombinant Mouse LAG3

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant extracellular domain (D1-D4) of Mouse LAG3 fused to the Fc portion of Mouse IgG2a.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LAG3 in Hut-78 cell lysate.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant Mouse LAG3 protein..

Affinity Binding Assay:: A representative lot of this antibody bound recombinant Mouse LAG3 protein with a KD of 3.2 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lymphocyte activation gene 3 protein (UniProt: Q61790; LAG-3, Activation-induced cytidine deaminase-linked autoimmunity protein, Aida, CD223) is encoded by the Lag3 gene (Gene ID: 16768) in murine species. LAG3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-442), a transmembrane domain (aa 443-463), and a cytoplasmic domain (aa 464-521). It contains three Ig-like C2-type domains and one Ig-like V-type domain. LAG3 is primarily expressed in activated CD4+ and CD8+ T-cells. Its expression is also observed in a subset of regulatory T cells. It serves as an inhibitory receptor on antigen activated T-cells  and deliver inhibitory signals upon binding to ligands, such as Fibrinogen-like protein 1 (FGL1). It can negatively regulate the proliferation, activation, effector function and homeostasis of both CD8+ and CD4+ T-cells. Following TCR engagement, it associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. LAG3 can be proteolytically cleaved by ADAM10 and ADAM17 within the connecting peptide region to generate a secretory form (sLAG3). ADAM10 is reported to mediate constitutive cleavage, but cleavage increases following T-cell activation. On the other hand, cleavage by ADAM17 is induced by TCR signaling in a protein kinase C theta (PRKCQ)-dependent manner. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Li, N., et al. (2007). EMBO J. 26(2); 494-504; Workman, CJ., et al. (2002). Eur. J. Immunol. 32(8); 2255-2263).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable