SRP6285

PCSK9 human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

• Synonym(s): FNDC5 (cleaved), Fibronectin Type III Domain-containing Protein 5 (cleaved), Fibronectin Type III Repeat-containing Protein 2 (cleaved)
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 75.1 kDa; observed mol wt 20 kDa; observed mol wt 62 kDa
• packaging: pkg of 20 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: Q8NBP7
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PCSK9(255738)

Description

General description

DTT-reduced. Protein migrates due to glycosylation and proteolytic digestion.Gln 31 is the predicted N-terminus.

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Physical form

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3