Derived from a tumour induced in an NZC mouse by irradiation, the cell line represents an early stage of B cell development characterised by high-density membrane immunoglobulin expression (IgGM, kappa) but no detectable immunoglobulin secretion.
Maintain cultures between 5x10,000 to 2x100,000 cells/ml; 5% CO2: 37°C.
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