This product is available in a 20 mL or 50 mL package size. The entire vial should be reconstituted in DI water as indicated by the package size. Each test requires 1 mL of this reagent. The 20 mL package size is sufficient to perform 20 test and the 50 mL pack size is sufficient to perform 50 tests.
G3293
Glucose Assay Reagent
for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20
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20 ML
$67.60
50 ML
$118.00
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20 ML
$67.60
50 ML
$118.00
About This Item
UNSPSC Code:
12164500
NACRES:
NA.84
Recommended Products
Quality Level
analyte chemical class(es)
sugars (glucose)
technique(s)
photometry: suitable
impurities
≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Specificity
< 0.05 % Activity resulting from cross-reactivity with the following substrates: fructose, dextran, sedoheptulose, arabinose, galactose, xylose, trehalose, mannose, sorbitol, +/- fucose or ribose.
Application
For the quantitative determination of glucose in food and other materials utilizing the hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay.
Reconstitute with deionized water using volume indicated by package size (20 or 50 mL).
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
To standardize an enzymatic assay procedure of cellulase.
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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For how many tests this reagent will be sufficient?
1 answer-
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How much of the initial must be reconstituted in 20 ml of DI water?
1 answer-
The entire vial should be reconstituted to the volume of the package size. The G3293-20 mL with 20 mL of DI water and the G3293-50 mL with 50 mL of DI water.
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Is magnesium required for Product G3293, Glucose Assay Reagent?
1 answer-
Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.
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Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?
1 answer-
In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.
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What is the detection limit of Product G3293, Glucose Assay Reagent?
1 answer-
This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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