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NYLM-RO

Roche

Nylon Membranes, positively charged

roll W × L 0.3 m × 3 m, sheet W × L 10 cm × 15 cm, sheet W × L 20 cm × 30 cm

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About This Item

UNSPSC Code:
41105339

packaging

pkg of 10 sheets (11209272001[20 x 30 cm])
pkg of 20 sheets (11209299001[10 x 15 cm])

Quality Level

manufacturer/tradename

Roche

roll W × L

0.3 m × 3 m

sheet W × L

10 cm × 15 cm
20 cm × 30 cm

storage temp.

20-25°C

General description

Microporous nylon 66 membrane on a polyester support, carrying positively charged (pH 3 - 10)  quaternary ammonium groups.
Surface Properties: Pore size of  0.45μm

Application

  • The physical and chemical properties of the nylon membranes make them specifically useful as a matrix for hybridization with nonradioactively (e.g., digoxigenin or biotin) and radioactively (e.g., [32P], [35S], an [3H])-labeled DNA, RNA, or oligonucleotide probes: Southern blots
  • Northern blots
  • Dot blots
They were developed to give the strongest signals and lowest background in color or chemiluminescent detection of digoxigenin-labeled hybrids.

Features and Benefits

Microporous nylon 66 membrane on a polyester support, carrying positively charged quaternary ammonium groups.

Contents: Membranes

Quality

Function tested for homogenous DNA detection in Southern blot procedures using the DIG System.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Certificates of Analysis (COA)

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Majid Kheirollahi et al.
Medical oncology (Northwood, London, England), 28(3), 864-870 (2010-04-08)
Telomeres at the ends of human chromosomes consist of tandem hexametric (TTAGGG)n repeats, which protect them from degradation. At each cycle of cell division, most normal somatic cells lose approximately 50-100 bp of the terminal telomeric repeat DNA. Precise prediction of
Araceli Nora García et al.
BMC plant biology, 14, 248-248 (2014-09-18)
The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological
Dervla T Isaac et al.
Infection and immunity, 81(2), 411-420 (2012-11-28)
Histoplasma capsulatum is a fungal respiratory pathogen that survives and replicates within the phagolysosome of macrophages. The molecular factors it utilizes to subvert macrophage antimicrobial defenses are largely unknown. Although the ability of H. capsulatum to prevent acidification of the
Stefanie Wehner et al.
RNA biology, 11(11), 1467-1478 (2014-12-09)
6S RNA is a highly abundant small non-coding RNA widely spread among diverse bacterial groups. By competing with DNA promoters for binding to RNA polymerase (RNAP), the RNA regulates transcription on a global scale. RNAP produces small product RNAs derived
Jes-Niels Boeckel et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(8), 3276-3281 (2011-02-09)
JmjC domain-containing proteins play a crucial role in the control of gene expression by acting as protein hydroxylases or demethylases, thereby controlling histone methylation or splicing. Here, we demonstrate that silencing of Jumonji domain-containing protein 6 (Jmjd6) impairs angiogenic functions

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