HPLC Analysis of Pegylated Exenatide on Zenix^® SEC-300 and Zenix^®-C SEC-300

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)

column

Zenix-C SEC-300, 30 cm x 7.8 mm, I.D., 3 μm particles (Z777092)

mobile phase

(A) 50 mM Ammonium acetate: (B) acetonitrile; (90:10, A:B, v/v)

flow rate

0.5 mL/min

pressure

609 psi (42 bar)

column temp.

25 °C

detector

UV, 214 nm

injection

15 μL

sample

3.3 mg/ml PEG-Exanatide in water (PEG 23 kD)