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  • Development and validation of an OECD reproductive toxicity test guideline with the mudsnail Potamopyrgus antipodarum (Mollusca, Gastropoda).

Development and validation of an OECD reproductive toxicity test guideline with the mudsnail Potamopyrgus antipodarum (Mollusca, Gastropoda).

Chemosphere (2017-05-05)
Katharina Ruppert, Cornelia Geiß, Clare Askem, Rachel Benstead, Rebecca Brown, Maira Coke, Virginie Ducrot, Philipp Egeler, Henrik Holbech, Thomas H Hutchinson, Karin L Kinnberg, Laurent Lagadic, Gareth Le Page, Ailbhe Macken, Peter Matthiessen, Sina Ostermann, Agnes Schimera, Claudia Schmitt, Anne Seeland-Fremer, Andy J Smith, Lennart Weltje, Jörg Oehlmann
ABSTRACT

Mollusks are known to be uniquely sensitive to a number of reproductive toxicants including some vertebrate endocrine disrupting chemicals. However, they have widely been ignored in environmental risk assessment procedures for chemicals. This study describes the validation of the Potamopyrgus antipodarum reproduction test within the OECD Conceptual Framework for Endocrine Disrupters Testing and Assessment. The number of embryos in the brood pouch and adult mortality serve as main endpoints. The experiments are conducted as static systems in beakers filled with artificial medium, which is aerated trough glass pipettes. The test chemical is dispersed into the medium, and adult snails are subsequently introduced into the beakers. After 28 days the reproductive success is determined by opening the brood pouch and embryo counting. This study presents the results of two validation studies of the reproduction test with eleven laboratories and the chemicals tributyltin (TBT) with nominal concentrations ranging from 10 to 1000 ng TBT-Sn/L and cadmium with concentrations from 1.56 to 25 μg/L. The test design could be implemented by all laboratories resulting in comparable effect concentrations for the endpoint number of embryos in the brood pouch. After TBT exposure mean EC

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cadmium sulfate hydrate, ≥99.995% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Tributyltin chloride, 96%
Pricing and availability is not currently available.