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  • High-performance liquid chromatographic enantioseparation of monoterpene-based 2-amino carboxylic acids on macrocyclic glycopeptide-based phases.

High-performance liquid chromatographic enantioseparation of monoterpene-based 2-amino carboxylic acids on macrocyclic glycopeptide-based phases.

Journal of chromatography. A (2010-09-25)
László Sipos, István Ilisz, Zoltán Pataj, Zsolt Szakonyi, Ferenc Fülöp, Daniel W Armstrong, Antal Péter
ABSTRACT

The enantiomers of five monoterpene-based 2-amino carboxylic acids were directly separated on chiral stationary phases containing macrocyclic glycopeptide antibiotics such as teicoplanin (Astec Chirobiotic T and T2) and teicoplanin aglycone (Chirobiotic TAG) as chiral selectors. The effects of pH, the mobile phase composition, the structure of the analyte and temperature on the separations were investigated. Experiments were performed at constant mobile phase compositions in the temperature range 10-40°C to study the effects of temperature and thermodynamic parameters on separations. Apparent thermodynamic parameters and T(iso) values were calculated from plots of ln k or ln α versus 1/T. Some mechanistic aspects of the chiral recognition process are discussed with respect to the structures of the analytes. It was found that the enantioseparations were in most cases enthalpy driven. The sequence of elution of the enantiomers was determined in all cases.

MATERIALS
Product Number
Brand
Product Description

Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Guard Column, Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Guard, Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Pricing and availability is not currently available.
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Pricing and availability is not currently available.