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  • Size exclusion chromatography of cellulose in LiCl/N,N-dimethylacetamide.

Size exclusion chromatography of cellulose in LiCl/N,N-dimethylacetamide.

Journal of biochemical and biophysical methods (2003-07-02)
Matija Strlic, Jana Kolar
ABSTRACT

Size exclusion of cellulose in LiCl/N,N-dimethylacetamide has been used for the past 15 years, yet much of the current research is still devoted to fundamental studies, as many issues regarding column calibration, separation mechanisms and solution behavior have not been resolved yet. The paper reviews practical aspects of sample preparation and it is demonstrated that sample heating and several techniques to aid solvent exchange call for reevaluation. It is further shown that the use of internal standard may introduce minor improvements in repeatability. The commonly used column calibration procedures, chromatographic conditions and applications are also reviewed. Further research is needed to understand the mechanisms of separation, to optimize column calibration and to facilitate and optimize sample preparation.

MATERIALS
Product Number
Brand
Product Description

USP
Residual Solvent Class 2 - N,N-Dimethylacetamide, United States Pharmacopeia (USP) Reference Standard
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N,N-Dimethylacetamide, ReagentPlus®, 99%
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N,N-Dimethylacetamide, spectrophotometric grade, ≥99%
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N,N-Dimethylacetamide, puriss. p.a., ≥99.5% (GC)
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Sigma-Aldrich
N,N-Dimethylacetamide, suitable for HPLC, ≥99.9%
Pricing and availability is not currently available.