Skip to Content
Merck

Biosynthesis of the halogenated auxin, 4-chloroindole-3-acetic acid.

Plant physiology (2012-05-11)
Nathan D Tivendale, Sandra E Davidson, Noel W Davies, Jason A Smith, Marion Dalmais, Abdelhafid I Bendahmane, Laura J Quittenden, Lily Sutton, Raj K Bala, Christine Le Signor, Richard Thompson, James Horne, James B Reid, John J Ross
ABSTRACT

Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Indole-3-pyruvic acid, ≥97%
SKU
Pack Size
Availability
Price
Quantity