- para-aminobenzoate synthesis from chorismate occurs in two steps.
para-aminobenzoate synthesis from chorismate occurs in two steps.
The Journal of biological chemistry (1989-05-25)
B P Nichols, A M Seibold, S Z Doktor
PMID2656685
ABSTRACT
Escherichia coli p-aminobenzoate synthase is composed of two nonidentical subunits encoded by pabA and pabB and has been assumed to be the sole enzyme responsible for p-aminobenzoate biosynthesis from chorismate and glutamine. Plasmids were constructed that overproduce the p-aminobenzoate synthase subunits 250-500-fold. Partial purification of the subunits revealed that they form a diffusible intermediate that is subsequently converted to p-aminobenzoate by a second enzyme (Mr = 49,000) temporarily designated enzyme X.
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