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  • Monocyte differentiation in localized juvenile periodontitis is skewed toward the dendritic cell phenotype.

Monocyte differentiation in localized juvenile periodontitis is skewed toward the dendritic cell phenotype.

Infection and immunity (2002-05-16)
Suzanne E Barbour, Yuichi Ishihara, Mohammed Fakher, Salma Al-Darmaki, Timothy H Caven, C P Shelburne, Al M Best, Harvey A Schenkein, John G Tew
ABSTRACT

Several lines of evidence indicate that the monocytes of subjects with localized juvenile periodontitis (LJP) are functionally distinct from cells of age- and race-matched nonperiodontitis (NP) subjects. Among the abnormalities are the propensity to secrete large amounts of prostaglandin E(2) and the induction of immunoglobulin G2 (IgG2) antibodies. The experiments described here were performed to further characterize the LJP monocytes and to determine if these cells mature differently than NP monocytes. When adherent monocytes from LJP subjects were cultured in the presence of human serum, both macrophages and cells with the morphology of immature monocyte-derived dendritic cells (MDDC) were observed. Within 4 days the prevalence of the immature MDDC was approximately twofold higher in LJP cultures than in NP cultures. In addition to their dendritic morphology, these cells were CD11c(+) and CD14(-) or CD14(low) and stimulated potent autologous mixed leukocyte reactions, consistent with differentiation to the MDDC phenotype. Like LJP monocytes, cultures of MDDC generated with interleukin-4 and granulocyte-macrophage colony-stimulating factor selectively induced IgG2 in cultures of pokeweed mitogen-stimulated NP leukocytes. Together, these data suggest that the monocytes of LJP subjects have a propensity to differentiate into MDDC and that this differentiation may be related to the high levels of IgG2 that are observed in the sera of LJP subjects. As high levels of circulating IgG2 are correlated with less severe disease, the propensity of LJP monocytes to differentiate into MDDC may have important implications for both the host response against oral pathogens and the progression of LJP.

MATERIALS
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Product Description

Sigma-Aldrich
Mouse Anti-Human IgG2 Antibody, clone HP6002, Fc, clone HP6002, Chemicon®, from mouse
Pricing and availability is not currently available.
Sigma-Aldrich
Mouse Anti-Human IgG1 Antibody, clone HP6001, Fc, clone HP6001, Chemicon®, from mouse
Pricing and availability is not currently available.