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  • Mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1.

Mechanisms governing the pioneering and redistribution capabilities of the non-classical pioneer PU.1.

Nature communications (2020-01-23)
Julia Minderjahn, Andreas Schmidt, Andreas Fuchs, Rudolf Schill, Johanna Raithel, Magda Babina, Christian Schmidl, Claudia Gebhard, Sandra Schmidhofer, Karina Mendes, Anna Ratermann, Dagmar Glatz, Margit Nützel, Matthias Edinger, Petra Hoffmann, Rainer Spang, Gernot Längst, Axel Imhof, Michael Rehli
ABSTRACT

Establishing gene regulatory networks during differentiation or reprogramming requires master or pioneer transcription factors (TFs) such as PU.1, a prototype master TF of hematopoietic lineage differentiation. To systematically determine molecular features that control its activity, here we analyze DNA-binding in vitro and genome-wide in vivo across different cell types with native or ectopic PU.1 expression. Although PU.1, in contrast to classical pioneer factors, is unable to access nucleosomal target sites in vitro, ectopic induction of PU.1 leads to the extensive remodeling of chromatin and redistribution of partner TFs. De novo chromatin access, stable binding, and redistribution of partner TFs both require PU.1's N-terminal acidic activation domain and its ability to recruit SWI/SNF remodeling complexes, suggesting that the latter may collect and distribute co-associated TFs in conjunction with the non-classical pioneer TF PU.1.

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Di(N-succinimidyl) glutarate, ≥97.0% (CHN)
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EN300-28333-100MG100 mg
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EN300-28333-250MG250 mg
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$280.80
EN300-28333-500MG500 mg
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EN300-28333-1G1 g
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$686.40
EN300-28333-2.5G2.5 g
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$1,344.20
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Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
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EN300-28333-100MG100 mg
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$196.30
EN300-28333-250MG250 mg
Ships in 7 days - from Aldrich Partner.
$280.80
EN300-28333-500MG500 mg
Ships in 7 days - from Aldrich Partner.
$529.10
EN300-28333-1G1 g
Ships in 7 days - from Aldrich Partner.
$686.40
EN300-28333-2.5G2.5 g
Ships in 7 days - from Aldrich Partner.
$1,344.20
Sigma-Aldrich
Anti-Actin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
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EN300-28333-100MG100 mg
Ships in 7 days - from Aldrich Partner.
$196.30
EN300-28333-250MG250 mg
Ships in 7 days - from Aldrich Partner.
$280.80
EN300-28333-500MG500 mg
Ships in 7 days - from Aldrich Partner.
$529.10
EN300-28333-1G1 g
Ships in 7 days - from Aldrich Partner.
$686.40
EN300-28333-2.5G2.5 g
Ships in 7 days - from Aldrich Partner.
$1,344.20
Sigma-Aldrich
PFI-3, ≥98% (HPLC)
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SKUVendor SKUPack SizeAvailabilityPriceQuantity
EN300-28333-100MG100 mg
Ships in 7 days - from Aldrich Partner.
$196.30
EN300-28333-250MG250 mg
Ships in 7 days - from Aldrich Partner.
$280.80
EN300-28333-500MG500 mg
Ships in 7 days - from Aldrich Partner.
$529.10
EN300-28333-1G1 g
Ships in 7 days - from Aldrich Partner.
$686.40
EN300-28333-2.5G2.5 g
Ships in 7 days - from Aldrich Partner.
$1,344.20