Chromatography
![Vantage® columns A set of five chromatography columns with varying heights, each featuring a clear middle section, black bases and tops, and some with blue valves.](/deepweb/assets/sigmaaldrich/marketing/global/images/categories/pharma-and-biopharma-manufacturing/vantage-column-1/vantage-column-1.png)
Downstream purification in bioprocessing includes one or more chromatographic separation steps to purify your molecule and remove impurities. Our broad chromatography portfolio includes resins, membranes, columns, and systems that work seamlessly together to simplify your workflow from process development to production scale.
Backed by unparalleled applications expertise and comprehensive support, we provide the confidence you need to tackle any chromatography challenge in your downstream process to increase productivity and improve efficiency.
![Evolving Regulations A complex industrial machine with various tubes, dials, and components, predominantly silver with blue accents and a logo featuring the letter ‘M’ on the side panel.](/deepweb/assets/sigmaaldrich/marketing/global/images/categories/pharma-and-biopharma-manufacturing/evolving-regulations/evolving-regulations.jpg)
Downstream Process & Formulation Chemicals
Downstream and formulation chemicals ensure the yield and safety of your drug product throughout the downstream process. Supporting high-risk applications, our chemicals are multi-compendial and manufactured according to GMP guidelines.
Ion Exchange Chromatography Resins
Ion exchange (IEX) chromatography separates biomolecules based on difference in charge between the target molecule and chromatography resin. Widely used in bioprocessing, our anion and cation exchange chromatography resins efficiently purify mAbs, recombinant proteins, vaccines, plasma, and other therapeutic molecules.
![Eshmuno A, affinity resin Set of ion exchange chromatography resin containers of varying sizes, with the largest on the right displaying a blue diamond-shaped hazard symbol, and two smaller bottles in front with white labels and blue text, all against a white background.](/deepweb/assets/sigmaaldrich/marketing/global/images/categories/pharma-and-biopharma-manufacturing/eshmuno-a-affinity-resin/eshmuno-a-affinity-resin.jpg)
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Single-use and multi-use chromatography systems are critical for the separation of your valuable molecules. We provide standard and custom systems from pilot to process-scale, offering robust and consistent performance with the processing flexibility you need.
Chromatography columns are critical to the successful separation of your valuable...
Affinity chromatography is often used during the capture phase...
Size exclusion chromatography (SEC) is a purification method used for separating substances...
Membrane chromatography is a highly productive downstream purification technology used for capture through polishing. Natrix® chromatography membrane is a single-use solution which improves process economics and facility utilization when compared to traditional membrane adsorbers.
High-performance liquid chromatography (HPLC) is a crucial step in the separation...
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