STRN250

Spectrum^™ Plant Total RNA Kit

sufficient for 250 purifications

• Synonym(s): Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
• UNSPSC Code: 41105501
• NACRES: NA.52

Properties

• usage: sufficient for 250 purifications
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): RNA purification: suitable
• greener alternative category: Aligned,
• storage temp.: 15-25°C

Description

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum^®Plant Total RNA Kit has been used:

• to extract RNA from the material ground in liquid nitrogen
• to isolate total RNA from mutant and wt (wild type) plantlets
• to extract total RNA from leaf samples
• to extract RNA from all plant tissues

Features and Benefits

• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.

Spectrum is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331,H310,H315,H317,H318,H361d,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Target Organs: Liver,Heart
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials