GE28-9365-52
HiPrep™ IMAC Fast Flow 16/10
Cytiva 28-9365-52
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About This Item
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packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9365-52
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Medium uncharged with metal ions.)
working range
3-12
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General description
Application
IMAC Sepharose™ 6 Fast FLow consists of 90 ?m beads of highly cross-linked Agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target Protein of interest. The bead size of IMAC Sepharose™ 6 Fast FLow enables high flow properties, making the medium an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged Proteins at process scale. Low metal ion leakage means that the activity of the purified Protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target Protein. Leakage of metal ions into the eluted target Protein pool from IMAC Sepharose™ 6 Fast FLow is generally Low under normal conditions.
IMAC Sepharose™ 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.
The medium is available prepacked in HiTrap® HiScreen™ and HiPrep™ column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep™ IMAC FF 16/10 columns. Prepacked HiScreen™ IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.
Features and Benefits
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Prepacked 20 mL HiPrep™ columns for easy scale-up.
- High binding capacity.
Storage and Stability
Analysis Note
Other Notes
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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