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product name
PSA1, 85011403
biological source
mouse embryo
growth mode
Suspension
karyotype
n = 40
morphology
Teratocarcinoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse SIM embryo teratocarcinoma
Cell Line Description
Thioguanine and ouabain resistant derivative of SIM. When cultured in absence of STO feeder cells it is stimulated to develop cell aggregates which differentiate resulting in formation of layer of endodermal cells covering aggregates.
Application
Differentiation and tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Requires STO feeder layer.
Other Notes
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Certificates of Analysis (COA)
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