1.46181

Rappaport Vassiliadis Medium

ready-to-use, tube volume 10 mL , Filling Volume

• Synonym(s): RVS Medium; Salmonella Enrichment Medium acc. to Rappaport and Vassiliadis
• UNSPSC Code: 41106225
• NACRES: NA.74

Properties

• Agency: EP 2.6.13; USP 62
• Quality Level: 300
• description: For the selective enrichment of samples for Salmonella sp. in accordance to EP
• form: liquid (ready-to-use)
• feature: closure type screw cap with septum; ready-to-use
• packaging: single packed of (10 ml in 17 ml tube with yellow cap (20 or 100 tubes per box)); tube of
• technique(s): direct inoculation: suitable; sterile filtration: suitable
• tube capacity: 17 mL
• tube volume: 10 mL , Filling Volume
• pH: 5.2
• application(s): microbiology; pharmaceutical
• storage temp.: 2-25°C
• suitability: Salmonella spp.

Description

General description

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. This medium is a modification of Rappaport-Vassiliadis enrichment broth and is used for motility enrichment in Petri dishes. It is a rapid and sensitive medium for the isolation of Salmonella sp. and can be seeded directly after pre-enrichment.

Application

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. in pharmaceutical products.

Analysis Note

Soy Peptone provides nitrogenous, carbonaceous, B complex vitamins and other essential growth nutrients. Phosphate provides buffering capacity to the medium. Malachite green serves as a selective agent. Sodium chloride maintains the osmotic equilibrium of the media. Salmonella generally survives a slightly high osmotic pressure (due to MgCl₂ in the medium), grows at a slightly low pH, and is resistant to malachite green compared to other bacteria, thus making it a selective media for the growth of Salmonella sp.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable