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  • Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.

Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.

Journal of nanoscience and nanotechnology (2014-04-18)
Lin Luo, Zhao Liu, Jianjun Li, Jian Zhu
ABSTRACT

A novel immunosensor of human immune globulin (IgG) was fabricated based on the fluorescence transfer between luminescent semiconductor quantum dots (QDs) and gold nanoparticles (AuNPs). AuNPs and CdSe/ZnS QDs were respectively labeled with immune reaction pair:IgG and goat anti-human immunoglobulin (GIgG), by optimizing the conditions including pH value and protein amount. In the assembled QD-GIgG-IgG-AuNP fluorescence resonance energy transfer (FRET) immunocomplex system, the presence of AuNP-IgG directly reduced the fluorescence intensity of the GIgG conjugated QDs. As a result, the concentration of AuNP-IgG had a linear relationship with the fluorescence decrease in a range of 0-1.57 microg/mL. Furthermore, the mechanism of the QDs' fluorescence decay has also been discussed and attributed to the light-induced photobleaching. This novel sensing method achieves quantitative detection of trace proteins, suggesting the potential of biomolecule-AuNPs conjugation based analytical methods in further application.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
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Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
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Gold, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, removable support, 99.99+%
Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
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Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, rod, 10mm, diameter 5.0mm, as drawn, 99.95%
Gold, microleaf disks, 10mm, thinness 1.0μm, specific density 1932μg/cm2, removable support, 99.99+%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.009mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, tube, 100mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, tube, 100mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, rod, 100mm, diameter 1.6mm, as drawn, 99.95%