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  • Plant cell wall engineering: applications in biofuel production and improved human health.

Plant cell wall engineering: applications in biofuel production and improved human health.

Current opinion in biotechnology (2014-04-01)
Rachel A Burton, Geoffrey B Fincher
ABSTRACT

Plant cell walls consist largely of cellulose, non-cellulosic polysaccharides and lignin. Concerted attempts are underway to convert wall polysaccharides from crop plant residues into renewable transport fuels and other valuable products, and to exploit the dietary benefits of cereal grain wall polysaccharides in human health. Attempts to improve plant performance for these applications have involved the manipulation of the levels and structures of wall components. Some successes in altering non-cellulosic polysaccharides has been achieved, but it would appear that drastic changes in cellulose are more difficult to engineer. Nevertheless, future prospects for both genetically modified (GM) and non-GM technologies to modify plant cell wall composition and structure remain bright, and will undoubtedly find applications beyond the current focus on human health and biofuel production.

MATERIALS
Product Number
Brand
Product Description

Supelco
Avicel® PH-101, ~50 μm particle size
Sigma-Aldrich
Sigmacell Cellulose, Type 101, Highly purified, fibers
Sigma-Aldrich
Sigmacell Cellulose, Type 20, 20 μm
Sigma-Aldrich
α-Cellulose, powder
Sigma-Aldrich
α-Cellulose, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Sigmacell Cellulose, Type 50, 50 μm
Supelco
Cellulose, DFS-0, microcrystalline, suitable for thin layer chromatography (TLC)
Sigma-Aldrich
Avicel® PH-101, tested according to Ph. Eur.
Supelco
Cellulose, powder, for column chromatography
Supelco
Cellulose, acid washed, powder, for column chromatography
Supelco
Cellulose, DS-0, powder, suitable for thin layer chromatography (TLC)
Sigma-Aldrich
Cellulose, microcrystalline, powder
Sigma-Aldrich
Cellulose, fibers, (medium)
Supelco
Cellulose, acid washed, from spruce, for column chromatography
Sigma-Aldrich
Cellulose, colloidal, microcrystalline
Sigma-Aldrich
Cellulose, microcrystalline, powder, 20 μm