Skip to Content
Merck
  • Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly Through Suppressing Nfatc1 Expression.

Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly Through Suppressing Nfatc1 Expression.

Biology (2020-08-13)
Kyubin Lee, You-Jee Jang, Hyerim Lee, Eunbin Kim, Yeojin Kim, Tong-Kewn Yoo, Tae Kyung Hyun, Jae-Il Park, Sun-Ju Yi, Kyunghwan Kim
ABSTRACT

Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In this study, we present the anti-osteoporotic activity of A. distichum extract by inhibiting osteoclast formation. First, we show that the methanolic extract of the leaves of A. distichum, but not extracts of the branches or fruits, significantly inhibits receptor activator of the NF-κB ligand (RANKL)-induced osteoclast differentiation. Second, our transcriptome analysis revealed that the leaf extract (LE) blocks sets of RANKL-mediated osteoclast-related genes. Third, the LE attenuates the phosphorylation of extracellular signal-related kinase. Finally, treatment with the LE effectively prevents postmenopausal bone loss in ovariectomized mice and glucocorticoid-induced osteoporosis in zebrafish. Our findings show that the extract of A. distichum efficiently suppressed osteoclastogenesis by regulating osteoclast-related genes, thus offering a novel therapeutic strategy for osteoporosis.

MATERIALS
Product Number
Brand
Product Description

Roche
Cell Proliferation Kit I (MTT)
Sigma-Aldrich
Leukocyte Acid Phosphatase (TRAP) Kit, Kit formulated with all liquid reagents
Sigma-Aldrich
Anti-SAPK/JNK Rabbit pAb, liquid, Calbiochem®
Sigma-Aldrich
PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb, liquid, Calbiochem®