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TOYOPEARL® DEAE-650S Bulk Media

phase DEAE (diethylaminoethyl), bottle of 250 mL, 35 μm particle size

Synonym(s):

TOYOPEARL® DEAE-650C Bulk Media

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About This Item

UNSPSC Code:
47101511

description

DEAE-650S

product line

TOYOPEARL®

form

slurry

feature

counter ion Cl-

packaging

bottle of 250 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

methacrylate

matrix active group

DEAE (diethylaminoethyl) phase

particle size

35 μm

operating pH

2-10

capacity

0.10 meq/mL±0.02 meq/mL ion exchange capacity
 30 g/L±5 adsorption capacity (BSA)
 30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)

compatibility

mode of use weak anion exchange chromatography

separation technique

anion exchange

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General description

Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.

Application

TOYOPEARL® DEAE-650S columns are used as separation media and ion exchange resins.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Rafael Picorel et al.
Methods in molecular biology (Clifton, N.J.), 684, 105-112 (2010-10-21)
A method to isolate and purify CP43 and CP47 pigment-protein complexes from Photosystem (PS) II of higher plants is presented. The method has been developed in spinach, but it may also be valid for other plant species, since there is
Rafael Picorel et al.
Methods in molecular biology (Clifton, N.J.), 274, 129-135 (2004-06-10)
A single-column method to purify the CP43 and CP47 pigment-protein complexes of photo-system (PS)II from higher plants is presented. To validate the isolation procedure, three different species were used (Spinacea oleracea, Beta vulgaris, and Glycine max), and the procedure worked

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