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EZHPI

Millipore

Human Proinsulin ELISA Kit

measures and quantifies Proinsulin levels in 20 μL serum or plasma

Synonym(s):

Human Proinsulin Detection, Proinsulin ELISA

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Human Total Proinsulin ELISA, This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.

Quality Level

200

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

assay range

accuracy: 88-103%
linearity: 84-120%
sensitivity: 0.5 pM
standard curve range: 2-200 pM

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

NCBI accession no.

NM_000207.2

UniProt accession no.

P01308

application(s)

research use

detection method

colorimetric (450nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Proinsulin, the precursor of insulin and C-peptide, is made by the β cells of the pancreas. Proinsulin formed by the cleavage of pre-proinsulin contains B- and A-domains linked through the connecting domain. Proinsulin interacts with insulin receptor-A (IR-A) isoform present in the nervous system and exhibits antiapoptotic and neuroprotective effects in the developing and postnatal nervous system. Elevated levels of proinsulin serve as a marker for secretory β-cell dysfunction. Increased levels of this hormone are also observed in patients with type 2 diabetes and impaired glucose tolerance. This kit measures the intact and intermediate metabolic forms of human proinsulin.

Application

Human Total Proinsulin ELISA has been used to measure plasma, serum, eye, and retinal levels of human proinsulin.

Other Notes

Research Sub Category
Obesity
Metabolic Disorders
Research Category
Metabolism
20 μL sample volume 3 hour assay, room temperature

Disclaimer

For research use only. Not for use in diagnostic procedures.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Warning

Hazard Statements

H290,H317

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carolina Isiegas et al.
Investigative ophthalmology & visual science, 57(8), 3610-3618 (2016-07-09)
The induction of proinsulin expression by transgenesis or intramuscular gene therapy has been shown previously to retard retinal degeneration in mouse and rat models of retinitis pigmentosa (RP), a group of inherited conditions that result in visual impairment. We investigated
Michael A Weiss
The Journal of biological chemistry, 284(29), 19159-19163 (2009-04-28)
Insulin plays a central role in the regulation of vertebrate metabolism. The hormone, the post-translational product of a single-chain precursor, is a globular protein containing two chains, A (21 residues) and B (30 residues). Recent advances in human genetics have
Flora de Pablo et al.
Frontiers in molecular neuroscience, 11, 426-426 (2018-12-12)
Proinsulin was first identified as the primary translation product of the insulin gene in Donald Steiner's laboratory in 1967, and was the first prohormone to be isolated and sequenced. While its role as an insulin precursor has been extensively studied
Andreas Pfützner et al.
Journal of diabetes science and technology, 5(3), 784-793 (2011-07-05)
Insulin resistance (IR) and deterioration of beta-cell secretion are main features in the development of type 2 diabetes, which is reflected in increasing serum intact proinsulin levels in later disease stage. Introduction of stable assays that are able to distinguish
Alonso Sánchez-Cruz et al.
Cell death & disease, 13(4), 383-383 (2022-04-22)
Synaptic loss, neuronal death, and circuit remodeling are common features of central nervous system neurodegenerative disorders. Retinitis pigmentosa (RP), the leading cause of inherited blindness, is a group of retinal dystrophies characterized by photoreceptor dysfunction and death. The insulin receptor

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