XNACRL

Extract-N-Amp^™ Cellular RNA Lysis Buffer

Optimized for rapid cell lysis and RNA stabilization

• Synonym(s): RNA extraction buffer, RNA isolation buffer, RNA lysis buffer
• UNSPSC Code: 41106306

Properties

• Quality Level: 200
• form: liquid
• storage condition: OK to freeze (-10 to-25°C)
• greener alternative product characteristics: Waste Prevention; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• suitability: suitable for PCR (suitable for RT-qPCR)
• greener alternative category: DfS-Developed
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

We are committed to bringing you Greener Alternative Products, which belongs to one of the four categories of greener alternatives. This product is a Design for Sustainability (DfS) developed product. Click here to view its DfS scorecard.

Application

• The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
• qRT-PCR
• RT-LAMP
• NGS library preparation

Features and Benefits

• From cell lysis to qRT-PCR in 15 minutes
• High Throughput and automation-friendly
• No RNA purification or clean up necessary
• No columns, heat, or centrifugation necessary
• Compatible with both adherent and non-adherent mammalian cell types
• Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

Physical form

Liquid

Storage and Stability

Freezer (-10 to-25°C)

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Extract-N-AMP Cellular RNA Lysis buffer 5ML, 50ML

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable