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P5412

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

Synonym(s):

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Empirical Formula (Hill Notation):
C6H8N2
CAS Number:
95-54-5
Molecular Weight:
108.14
Beilstein:
606074
EC Number:
202-430-6
MDL number:
MFCD00007721
UNSPSC Code:
12352204
PubChem Substance ID:
24898638
NACRES:
NA.83

product name

o-Phenylenediamine, tablet, 20 mg substrate per tablet

vapor density

3.7 (vs air)

vapor pressure

0.01 mmHg ( 25 °C)

form

tablet

bp

256-258 °C

mp

100-102 °C

fluorescence

λex 337 nm; λem 417 nm (after derivatization)

storage temp.

2-8°C

SMILES string

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI key

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

276.8 °F - closed cup

Flash Point(C)

136 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Application of o-phenylenediamine as a fluorogenic substrate in peroxidase-mediated enzyme-linked immunosorbent assay
Mekler VM and Bystryak SM
Analytica Chimica Acta, 264(2), 359-363 (1992)
Interleukin-13 Regulates Secretion of the Tumor Growth Factor-beta Superfamily Cytokine Activin A in Allergic Airway Inflammation
Hardy CL, et al.
American Journal of Respiratory Cell and Molecular Biology, 42(6), 667-675 (2010)
Receptor-mediated abeta amyloid antibody targeting to Alzheimer?s disease mouse brain
Zhou QH, et al.
Molecular Pharmaceutics, 8(1), 280-285 (2010)
Jonas R Knudsen et al.
International journal of molecular sciences, 21(7) (2020-04-02)
The small molecule kinase inhibitor SBI-0206965 was originally described as a specific inhibitor of ULK1/2. More recently, it was reported to effectively inhibit AMPK and several studies now report its use as an AMPK inhibitor. Currently, we investigated the specificity
Fu Ci Guo et al.
Diseases of aquatic organisms, 61(1-2), 175-178 (2004-12-09)
The course of Spironucleus barkhanus (Diplomonadida: Hexamitidae) infection in Atlantic salmon Salmo salar L. (Salmonidae) has 2 distinct phases, a blood phase and a tissue phase. To detect and quantify an infection, 3 parasitological techniques, namely Wet Mount Examination (WME)
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