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A8229

Sigma-Aldrich

Monoclonal Anti-A2B5 antibody produced in mouse

clone 105, purified immunoglobulin, lyophilized powder

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About This Item

MDL number:
MFCD02095786
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

105, monoclonal

form

lyophilized powder

species reactivity

human, rat, chicken, mouse

technique(s)

flow cytometry: 2.5 μg using using retinoic acid-differentiated NTera-2 human testicular embryonic carcinoma cell line (per 106 cells)
immunocytochemistry: 8-25 μg/mL using using NTera-2 human cell line

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

A2B5 is a ganglioside that has recently been used as marker for stem cells.
Monoclonal Anti-A2B5, Clone 105, was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with embryonic chicken retinal cells.
Mouse Monoclonal Anti-A2B5 antibody binds to human, rat, chicken and mouse A2B5.

Immunogen

embryonic chicken retinal cells.

Application

Mouse Monoclonal Anti-A2B5 antibody can be used for flow cytometry (2.5μg) and immunocytochemistry (8-25μg/ml) assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C M Wen et al.
Journal of fish biology, 90(1), 201-221 (2016-10-13)
In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein
Nagako Kawashima et al.
Journal of neurochemistry, 111(4), 1031-1041 (2009-09-22)
Sandhoff disease is a progressive neurodegenerative disorder caused by mutations in the HEXB gene which encodes the beta-subunit of N-acetyl-beta-hexosaminidase A and B, resulting in the accumulation of the ganglioside GM2. We isolated astrocytes from the neonatal brain of Sandhoff
Mikko T Huuskonen et al.
The Journal of experimental medicine, 219(1) (2021-12-01)
Subcortical white matter (WM) stroke accounts for 25% of all strokes and is the second leading cause of dementia. Despite such clinical importance, we still do not have an effective treatment for ischemic WM stroke, and the mechanisms of WM
Cristina Martínez-Ramos et al.
Journal of tissue engineering and regenerative medicine, 13(3), 509-521 (2019-02-07)
Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and
Tomoko Yamashita et al.
PloS one, 12(2), e0171947-e0171947 (2017-02-14)
Oligodendrocytes myelinate axons and form myelin sheaths in the central nervous system. The development of therapies for demyelinating diseases, including multiple sclerosis and leukodystrophies, is a challenge because the pathogenic mechanisms of disease remain poorly understood. Primate pluripotent stem cell-derived
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