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Merck

Biochemical activities of 6-carboxy β-chitin derived from squid pens.

Carbohydrate polymers (2012-10-10)
Jun Huang, Wan-wen Chen, Sheng Hu, Jing-Yan Gong, Hong-Wu Lai, Peng Liu, Le-he Mei, Jian-wei Mao
ABSTRACT

TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated 6-carboxy β-chitin derivatives (T-chitin) with different carboxylate content were successfully synthesized by controlling the addition level of NaClO as the primary oxidant. The structural and biochemical properties of the derivatives were investigated. The carboxylate contents of the derivatives calculated by electrical conductivity titration were 1.33, 1.68, 1.80, and 2.08 mmol/g, respectively. The yield of T-chitin with carboxylate content of 2.08 mmol/g reached 74.55%. T-chitin exhibited stronger bile acid binding capacities than that of β-chitin. The scavenging ability of T-chitin against hydroxyl radicals improved with increasing concentration, and EC(50) values were below 1.2 mg/mL. All T-chitin exhibited a strong ferrous ion chelating effect. At 8 mg/mL, the chelating effects of T-chitin with carboxylate content of 0.81 mmol/g reached 80.15%. These results showed that T-chitin had good bile acid binding capacity and antioxidant activities and it may be a potential antioxidant in vitro.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium bromide, ReagentPlus®, ≥99%
Sigma-Aldrich
Sodium bromide, ACS reagent, ≥99.0%
Supelco
Density Standard 1251 kg/m3, H&D Fitzgerald Ltd. Quality
Sigma-Aldrich
Sodium bromide, BioXtra, ≥99.0%
Sigma-Aldrich
Sodium bromide, BioUltra, ≥99.0% (AT)
Sigma-Aldrich
Sodium bromide, ≥99.99% trace metals basis
Sigma-Aldrich
TEMPO, purified by sublimation, 99%
Sigma-Aldrich
TEMPO, 98%