A1226

Anti-Actopaxin antibody produced in rabbit

IgG fraction of antiserum, PBS solution

• Synonym(s): Anti-α-Parvin, Anti-CH-ILKBP
• MDL number: MFCD04040144
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: PBS solution
• mol wt: antigen 42 kDa
• species reactivity: human, mouse
• technique(s): microarray: suitable; western blot: 1:3,000 using whole cell extract of the human endothelial ECV304 cell line.
• UniProt accession no.: Q9NVD7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PARVA(55742) ; mouse ... Parva(57342)

Description

General description

Actopaxin is a member of actin binding proteins that include beta-parvin/affixin and gamma-parvin. Actopaxin is known to associate with F-actin and paxillin LD motifs LD1 and LD4. This protein also directly binds to serine/threonine integrin-linked kinase (ILK). Studies have reported that actopaxin may be involved in integrin-dependent remodeling of the actin cytoskeleton during cell adhesion, motility and division.
Rabbit anti-actopaxin recognizes actopaxin (42 kDa). Staining of actopaxin in immunoblotting is specifically inhibited with actopaxin immunizing peptide (mouse, amino acids 35-53).

Immunogen

synthetic peptide corresponding to the N-terminal region of mouse actopaxin (amino acids 35-53). The sequence is identical in human actopaxin and has considerable homology (~70%) with β-parvin/affixin, but no homology with γ-parvin.

Application

Actopaxin was detected in HEK 293 cell lysates at 42 kDa by western blot using rabbit anti-actopaxin antibody at a 1:1000 dilution. In these cells, actopaxin associated with an ILK complex.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Rabbit anti-actopaxin can also be used for protein microaarays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

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